Introduction of four transcription factors Oct-4, Sox2, Klf4 and c-Myc into adult cells can covert them to pluripotent stem cells. These induced pluripotent stem cells (iPSCs) exhibit features characteristic of embryonic stem (ES) cells. These cells have exciting new prospects for biomedical research, drug discovery and for regenerative medicine. Our lab is interested in understanding the mechanism of the reprogramming process and the use of these cells to understand the pathophysiology of human diseases.

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Selected Publications

• Raina K, Joshi G, Modak K, Premkumar C, Priyanka S, Rajesh P, Velayudhan SR,Thummer RP. Generation and characterization of induced pluripotent stem cell line IITGi001-A derived from adult human primary dermal fibroblasts. Stem Cell Res. 2023 Jun 28;71:103159. doi: 10.1016/j.scr.2023.103159.
• Rajamani BM, Illangeswaran RSS, Benjamin ESB, Balakrishnan B, Jebanesan DZP, Das S, Pai AA, Vidhyadharan RT, Mohan A, Karathedath S, Abraham A, Mathews V, Velayudhan SR, Balasubramanian P. Modulating retinoid-X-receptor alpha (RXRA)expression sensitizes chronic myeloid leukemia cells to imatinib in vitro and reduces disease burden in vivo. Front Pharmacol. 2023 May 31;14:1187066.
• Venkatesan V, Christopher AC, Rhiel M, Azhagiri MKK, Babu P, Walavalkar K, Saravanan B, Andrieux G, Rangaraj S, Srinivasan S, Karuppusamy KV, Jacob A, Bagchi A, Pai AA, Nakamura Y, Kurita R, Balasubramanian P, Pai R, Marepally SK, Mohankumar KM, Velayudhan SR, Boerries M, Notani D, Cathomen T, Srivastava A, Thangavel S. Editing the core region in HPFH deletions alters fetal and adult globin expression for the treatment of β-hemoglobinopathies. Mol Ther Nucleic Acids. 2023 Apr 26;32:671-688. doi: 10.1016/j.omtn.2023.04.024. PMID: 37215154; PMCID: PMC10197010.
• Benjamin ES, Babu B, Joshi G, Rajamani BM, Nandy K, Rani S, Anandhan S, PremKumar C, Maddali M, Abraham A, Velayudhan SR*, Balasubramanian P* (*Corresponding Authors) Inhibition of BCR::ABL1 tyrosine kinase activity Aids in the Generation of Stable Chronic Myeloid Leukemia Induced Pluripotent Stem Cells. bioRxiv.
• Illangeswaran RSS, Jebanesan DZP, Sivakumar KK, Vidhyadharan RT, Rajamani BM, Janet NB, David E, Velayudhan SR, Mathews V, Balasubramanian P. Chemotherapeutic drugs elicit stemness and metabolic alteration to mediate acquired drug-resistant phenotype in acute myeloid leukemia cell lines. Leuk Res. 2023 May;128:107054.
• Joshi G, Arthur NBJ, Geetha TS, Datari PVR, Modak K, Roy D, Chaudhury AD,Sundaraganesan P, Priyanka S, Na F, Ramprasad V, Abraham A, Srivastava VM,Srivastava A, Kulkarni UP, George B, Velayudhan SR. Comprehensive laboratory diagnosis of Fanconi anaemia: comparison of cellular and molecular analysis. J Med Genet. 2023 Mar 9:jmedgenet-2022-108714.
• George A, Ravi NS, Prasad K, Panigrahi L, Koikkara S, Rajendiran V, Devaraju N, Paul J, Pai AA, Nakamura Y, Kurita R, Balasubramanian P, Thangavel S, Marepally S, Velayudhan SR, Srivastava A, Mohankumar KM. Efficient and error-free correction of sickle mutation in human erythroid cells using prime editor-2. Front Genome Ed. 2022 Dec 20;4:1085111.
• Dahariya S, Raghuwanshi S, Thamodaran V, Velayudhan SR, Gutti RK. Role of Long Non-Coding RNAs in Human-Induced Pluripotent Stem Cells Derived Megakaryocytes: A p53, HOX Antisense Intergenic RNA Myeloid 1, and miR-125b Interaction Study. J Pharmacol Exp Ther. 2023 Jan;384(1):92-101.
• Bagchi A, Devaraju N, Chambayil K, Rajendiran V, Venkatesan V, Sayed N, Pai AA, Nath A, David E, Nakamura Y, Balasubramanian P, Srivastava A, Thangavel S,Mohankumar KM, Velayudhan SR. Erythroid lineage-specific lentiviral RNAi vectors suitable for molecular functional studies and therapeutic applications. Sci Rep.2022 Aug 18;12(1):14033.
• Das S, Stallon Illangeswaran RS, Ijee S, Kumar S, Velayudhan SR, Balasubramanian P. Pooled shRNA Library Screening to Identify Factors that Modulate a Drug Resistance Phenotype. J Vis Exp. 2022 Jun 17;(184).
• Mahalingam G, Rachamalla HK, Arjunan P, Periyasami Y, M S, Thangavel S, Mohankumar KM, Moorthy M, Velayudhan SR, Srivastava A, Marepally S. Optimization of SARS-CoV-2 Pseudovirion Production in Lentivirus Backbone With a Novel Liposomal System. Front Pharmacol. 2022 Mar 25;13:840727.
• Ravi NS, Wienert B, Wyman SK, Bell HW, George A, Mahalingam G, Vu JT, Prasad K, Bandlamudi BP, Devaraju N, Rajendiran V, Syedbasha N, Pai AA, Nakamura Y,Kurita R, Narayanasamy M, Balasubramanian P, Thangavel S, Marepally S, Velayudhan SR, Srivastava A, DeWitt MA, Crossley M, Corn JE, Mohankumar KM.Identification of novel HPFH-like mutations by CRISPR base editing that elevate the expression of fetal hemoglobin. Elife. 2022 Feb 11;11:e65421.
• Nath A, Rayabaram J, Ijee S, Bagchi A, Chaudhury AD, Roy D, Chambayil K,Singh J, Nakamura Y, Velayudhan SR. Comprehensive Analysis of microRNAs in Human Adult Erythropoiesis. Cells. 2021 Nov 4;10(11):3018.
• Christopher AC, Venkatesan V, Karuppusamy KV, Srinivasan S, Babu P, Azhagiri MKK, Chambayil K, Bagchi A, Rajendiran V, Ravi NS, Kumar S, Marepally SK, Mohankumar KM, Srivastava A, Velayudhan SR, Thangavel S. Preferential Expansion of Human CD34+CD133+CD90+ Hematopoietic Stem Cells Enhances Gene-Modified Cell Frequency for Gene Therapy. Hum Gene Ther. 2022 Feb;33(3-4):188-201.
• Singh G, Manian KV, Premkumar C, Srivastava A, Daniel D, Velayudhan SR.Derivation of Clinical-Grade Induced Pluripotent Stem Cell Lines from Erythroid Progenitor Cells in Xenofree Conditions. Methods Mol Biol. 2022;2454:775-789.
• Thamodaran V, Rani S, Velayudhan SR. Gene Editing in Human Induced Pluripotent Stem Cells Using Doxycycline-Inducible CRISPR-Cas9 System. Methods Mol Biol. 2022;2454:755-773.
• Bagchi A, Nath A, Thamodaran V, Ijee S, Palani D, Rajendiran V, Venkatesan V, Datari P, Pai AA, Janet NB, Balasubramanian P, Nakamura Y, Srivastava A,Mohankumar KM, Thangavel S, Velayudhan SR. Direct Generation of Immortalized Erythroid Progenitor Cell Lines from Peripheral Blood Mononuclear Cells. Cells. 2021 Mar 1;10(3):523.
• Benjamin ESB, Ravindra N, Rajamani BM, Anandan S, Kausalya B, Veldore V, Mathews V, Velayudhan SR, Balasubramanian P. BCR-ABL1 kinase domain mutation analysis by next-generation sequencing detected additional mutations in chronic myeloid leukemia patients with suboptimal response to imatinib. Leuk Lymphoma. 2021 Jun;62(6):1528-1531.
• Ravindra N, Athiyarath R, S E, S S, Kulkarni U, N A F, Korula A, Shaji RV, George B, Edison ES. Novel frameshift variant (c.409dupG) in SLC25A38 is a common cause of congenital sideroblastic anaemia in the Indian subcontinent. J Clin Pathol. 2021 Mar;74(3):157-162.
• Arunachalam AK, Suresh H, Edison ES, Korula A, Aboobacker FN, George B, Shaji RV, Mathews V, Balasubramanian P. Screening of genetic variants in ELANE mutation negative congenital neutropenia by next generation sequencing. J Clin Pathol. 2020 Jun;73(6):322-327.
• Sullivan S, Stacey GN, Akazawa C, Aoyama N, Baptista R, Bedford P, Bennaceur Griscelli A, Chandra A, Elwood N, Girard M, Kawamata S, Hanatani T, Latsis T, Lin S, Ludwig TE, Malygina T, Mack A, Mountford JC, Noggle S, Pereira LV, Price J, Sheldon M, Srivastava A, Stachelscheid H, Velayudhan SR, Ward NJ, Turner ML, Barry J, Song J. Quality control guidelines for clinical-grade human induced pluripotent stem cell lines. Regen Med. 2018 Oct;13(7):859-866.
• Manian KV, Bharathan SP, Maddali M, Srivastava VM, Srivastava A, Velayudhan SR. Generation of an integration-free iPSC line (CSCRi005-A) from erythroid progenitor cells of a healthy Indian male individual. Stem Cell Res. 2018 May;29:148-151.
• Fouzia NA, Edison ES, Lakshmi KM, Korula A, Velayudhan SR, Balasubramanian P, Abraham A, Viswabandya A, George B, Mathews V, Srivastava A. Long-term outcome of mixed chimerism after stem cell transplantation for thalassemia major conditioned with busulfan and cyclophosphamide. Bone Marrow Transplant. 2018 Feb;53(2):169-174.
• Karathedath S, Rajamani BM, Musheer Aalam SM, Abraham A, Varatharajan S, Krishnamurthy P, Mathews V, Velayudhan SR, Balasubramanian P. Role of NF-E2 related factor 2 (Nrf2) on chemotherapy resistance in acute myeloid leukemia (AML) and the effect of pharmacological inhibition of Nrf2. PLoS One. 2017 May 15;12(5):e0177227.
• Bharathan SP, Nandy K, Palani D, Janet A NB, Natarajan K, George B, Srivastava A, Velayudhan SR. Generation of an induced pluripotent stem cell line that mimics the disease phenotypes from a patient with Fanconi anemia by conditional complementation. Stem Cell Res. 2017 Apr;20:54-57.
• Dharmalingam P, Marrapu B, Voshavar C, Nadella R, Rangasami VK, Shaji RV, Abbas S, Prasad RBN, Kaki SS, Marepally S. An anti-oxidant, α-lipoic acid conjugated oleoyl-sn-phosphatidylcholineas a helper lipid in cationic liposomal formulations. Colloids Surf B Biointerfaces. 2017 Apr 1;152:133-142.
• Bharathan SP, Manian KV, Aalam SM, Palani D, Deshpande PA, Pratheesh MD, Srivastava A, Velayudhan SR. Systematic evaluation of markers used for the identification of human induced pluripotent stem cells. Biol Open. 2017 Jan 15;6(1):100-108.
• Srivastava A, Shaji RV. Cure for thalassemia major – from allogeneic hematopoietic stem cell transplantation to gene therapy. Haematologica. 2017 Feb;102(2):214-223.
• Aalam SM, Manian KV, Bharathan SP, Mayuranathan T, Velayudhan SR. Identification of Stable OCT4+NANOG- State in Somatic Cell Reprogramming. Cell Reprogram. 2016 Nov;18(6):367-368.
• Kamath MS, Pradhan S, Edison ES, Velayudhan SR, Antonisamy B, Karthikeyan M, Mangalaraj AM, Kunjummen A, George K. Chorionic villous sampling through transvaginal ultrasound approach: A retrospective analysis of 1138 cases. J Obstet Gynaecol Res. 2016 Oct;42(10):1229-1235.
• Karathedath S, Rajamani BM, Musheer Aalam SM, Abraham A, Varatharajan S, Krishnamurthy P, Mathews V, Shaji RV, Balasubramanian P. Role of NF-E2 related factor 2 (Nrf2) on chemotherapy resistance in acute myeloid leukemia (AML) and the effect of pharmacological inhibition of Nrf2. PLoS One. 2017 May 15;12(5):e0177227.
• Sumitha Prameela Bharathana, Krittika Nandy, Dhavapriya Palani, Nancy Beryl Janet A, Kasthuri Natarajan, Biju George, Alok Srivastava, Shaji RV. Generation of an induced pluripotent stem cell line that mimics the disease phenotypes from a patient with Fanconi anemia by conditional complementation. Stem Cell Research Volume 20, April 2017, Pages 54–57.
• Sumitha Prameela Bharathan, Kannan Vrindavan Manian, Syed Mohammed Musheer Aalam, Dhavapriya Palani, Prashant Ajit Deshpande, Mankuzhy Damodaran Pratheesh, Alok Srivastava, Shaji RV. Systematic evaluation of markers used for the identification of human induced pluripotent stem cells. Biology Open 2017 6: 100-108; doi: 10.1242/bio.022111.
• Srivastava A,Shaji RV. Cure for thalassemia major: from allogeneic hematopoietic stem cell transplantation to gene therapy. Haematologica 2017 Feb;102(2):214-223.
• Aalam SM, Manian KV, Bharathan SP, Mayuranathan T, Shaji RV. Identification of Stable OCT4+NANOG- State in Somatic Cell Reprogramming. Cell Reprogram. 2016 Nov;18(6):367-368.
• Deshpande P, Kathirvel K, Alex AA, Korula A, George B, Shaji RV, Mathews V. Leukocyte Adhesion Deficiency-I: Clinical and Molecular Characterization in an Indian Population. Indian J Pediatr. 2016 Aug;83(8):799-804.
• Kamath MS, Pradhan S, Edison ES, Shaji RV, Antonisamy B, Karthikeyan M, Mangalaraj AM, Kunjummen A, George K. Chorionic villous sampling through transvaginal ultrasound approach: A retrospective analysis of 1138 cases. J Obstet Gynaecol Res. 2016 Oct;42(10):1229-1235.
• Pal R, Mariappan I, Shaji RV. Editorial: Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells: Ushering of a New Era in Personalized Cell Therapies. Curr Stem Cell Res Ther. 2016;11(2):97-8.
• Manian KV, Aalam SM, Bharathan SP, Srivastava A, Shaji RV. Understanding the Molecular Basis of Heterogeneity in Induced Pluripotent Stem Cells. Cellular Reprogramming 2015 Dec;17(6):427-40.
• Jayasree D, Shaji RV, George B, Mathews V, Srivastava A, Edison ES. Clinical, Hematological and Molecular Analysis of Homozygous Hb E (HBB: c.79G?>?A) in the Indian Population. Hemoglobin. 2016;40(1):16-9.
• Abraham A, Varatharajan S, Karathedath S, Philip C, Lakshmi KM, Jayavelu AK, Mohanan E, Janet NB, Srivastava VM, Shaji RV, Zhang W, Abraham A, Viswabandya A, George B, Chandy M, Srivastava A, Mathews V, Balasubramanian P. RNA expression of genes involved in cytarabine metabolism and transport predicts cytarabine response in acute myeloid leukemia. Pharmacogenomics. 2015 Jul;16(8):877-90.
• Deshpande P, Kamalanathan N, Sampath E, George B, Shaji RV, Edison ES. Characterization of Clinical and Laboratory Profiles of the Deletional a2-Globin Gene Polyadenylation Signal Sequence (AATAAA?>?AATA-?-) in an Indian Population. Hemoglobin. 2015 Dec;39(6):415-8.
• Mayuranathan T, Rayabaram J, Das R, Arora N, Edison ES, Chandy M, Srivastava A, Shaji RV. Identification of rare and novel deletions that cause (dß)0-thalassaemia and hereditary persistence of foetal haemoglobin in Indian population. Eur J Haematol. 2014 Jun;92(6):514-20.
• Mayuranathan T, Rayabaram J, Edison ES, Srivastava A, Shaji RV. A novel deletion of ß-globin promoter causing high HbA2 in an Indian population Haematologica. 2012 Sep;97(9):1445-7.
• David S, Jayandharan GR, Abraham A, Jacob RR, Devi GS, Patkar N, Shaji RV, Nair SC, Viswabandya A, Ahmed R, George B, Mathews V, Chandy M, Srivastava A. Molecular basis of Wiskott-Aldrich syndrome in patients from India. Eur J Haematol. 2012 Oct;89(4):356-60.
• Varatharajan S, Abraham A, Zhang W, Shaji RV, Ahmed R, Abraham A, George B, Srivastava A, Chandy M, Mathews V, Balasubramanian P. Carbonyl reductase 1 expression influences daunorubicin metabolism in acute myeloid leukemia. Eur J Clin Pharmacol. 2012 Dec;68(12):1577-86.
• Jain S, Edison ES, Mathews V, Shaji RV. A novel d-globin gene mutation (HBD: c.323G>A) masking the diagnosis of ß-thalassemia: a first report from India. Int J Hematol. 2012 May;95(5):570-2.
• Edison ES, Sathya M, Rajkumar SV, Nair SC, Srivastava A, Shaji RV. A novel ß-globin gene mutation HBB.c.22 G>C produces a hemoglobin variant (Hb Vellore) mimicking HbS in HPLC. Int J Lab Hematol. 2012 Oct;34(5):556-8.
• Abraham A, Varatharajan S, Abbas S, Zhang W, Shaji RV, Ahmed R, Abraham A, George B, Srivastava A, Chandy M, Mathews V, Balasubramanian P. Cytidine deaminase genetic variants influence RNA expression and cytarabine cytotoxicity in acute myeloid leukemia. Pharmacogenomics. 2012 Feb;13(3):269-82.
• Edison ES, Venkatesan RS, Govindanattar SD, George B, Shaji RV. A novel 26 bp deletion [HBB: c.20_45del26bp] in exon 1 of the ß-globin gene causing ß-thalassemia major. Hemoglobin.2012;36(1):98-102.
• Edison ES, Shaji RV, Chandy M, Srivastava A. Interaction of hemoglobin E with other abnormal hemoglobins. Acta Haematol. 2011;126(4):246-8.
• Mathews V, George B, Chendamarai E, Lakshmi KM, Desire S, Balasubramanian P, Viswabandya A, Thirugnanam R, Abraham A, Shaji RV, Srivastava A, Chandy M. Single-agent arsenic trioxide in the treatment of newly diagnosed acute promyelocytic leukemia: long-term follow-up data. J Clin Oncol. 2010 Aug 20;28(24):3866-71.

Check the following link for the full list of publications

https://pubmed.ncbi.nlm.nih.gov/?term=velayudhan+sr

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