| MOLECULAR DIAGNOSIS OF LEUKEMIA |
| B5027 |
RTPCR for ALL
Qualitative analysis of fusion transcripts by Nested Reverse Transcriptase PCR (RT-PCR). Appropriate translocations are analyzed as required by clinical diagnosis and primary lab diagnosis.
Translocations Analyzed:
1. RTPCR for p210 BCR-ABL
2. RTPCR for p190 BCR-ABL
3. RTPCR for TEL-AML1,
4. RTPCR for MLL-AF4,
5. RTPCR for E2A-PBX
|
9 mL Peripheral blood or Bone marrow freshly drawn in EDTA (Purple top) tube. |
| B5045 |
RTPCR for APML / CML / AML / HES
Qualitative analysis of fusion transcripts by Nested Reverse Transcriptase PCR (RT-PCR). Appropriate translocations are analyzed as required by clinical diagnosis and primary lab diagnosis.
Translocations Analyzed:
1. RTPCR for p210 BCR-ABL for CML
2. RTPCR for p190 BCR-ABL for CML
3. RTPCR for PML-RARα (bcr 1,2,3) for APML
4. RTPCR for PLZF-RARα and NuMA-RARα for APML
5. RTPCR for AML-ETO for AML
6. RTPCR for Inversion-16 (CBFB-MYH11) for AML
7. RTPCR for FIP1L1-PDGFRA in hyper eosinophilic syndrome (HES)
|
9 mL Peripheral blood or Bone marrow freshly drawn in EDTA (Purple top) tube.
|
| B5059 |
ABL KINASE DOMAIN MUTATION
Screening of BCR-ABL Kinase Domain mutations by Sanger sequencing.
|
9 mL Peripheral blood or Bone marrow freshly drawn in EDTA (Purple top) tube. |
| B5053 |
REAL-TIME RT PCR (RQ PCR)
Quantitative analysis of fusion transcripts by quantitative real time PCR
(RNA Based)
1. RQ PCR - p210 (b3a2, b2a2) and P190 (e1a2) BCR-ABL fusion transcripts in CML
2. RQ PCR - p210 - p210 (b3a2, b2a2) and P190 (e1a2) BCR-ABL fusion transcripts in ALL
|
9 mL Peripheral blood or Bone marrow freshly drawn in EDTA (Purple top) tube. |
| B5057 |
LD PCR for BURKITT’S
Analysis for t(8:14) (cMYC-IgH) translocation in Burkitt’s Lymphoma by
Long distance PCR (DNA based)
|
9 mL Peripheral blood in EDTA (Purple top) tube. Stored at 4°C. |
| B5067 |
NPM1 & FLT3-ITD, FLT3-TKD Mutations for AML
1. NPM1 Exon 12 mutations and FLT3 - Internal tandem duplication (ITD) by multiplex PCR and gene scan.
2. FLT3 - Tyrosine Kinase domain mutation (TKD) by PCR and RFLP.
|
9 mL Peripheral blood in EDTA (Purple top) tube. Stored at 4°C. |
| B5072 |
C-KIT Mutations SCREENING
Mutations screening of C-KIT Exons 8 & 17 for AML and Systemic Mastocytosis. |
9 mL Peripheral blood in EDTA (Purple top) tube. Stored at 4°C. |
| B5090 |
MPN - Myeloproliferative Neoplasm Mutations panel
Panel of MPN Mutations done as required by clinical diagnosis and primary lab diagnosis:
1. JAK2 - V617F mutation screening by allele specific PCR.
2. JAK2 - exon12 mutation screening by DNA Sequencing
3. CALR mutation screening by PCR and Genescan
4. MPL mutations screening by allele specific PCR.
|
9 mL Peripheral blood in EDTA (Purple top) tube. Stored at 4°C. |
| B5091 |
BRAF Mutation analysis.
BRAF V600E mutation analysis for Hairy Cell Leukemia. |
9 mL Peripheral blood in EDTA (Purple top) tube. Stored at 4°C. |
| B5065 |
TPMT POLYMORPHISM SCREENING
The following TPMT Polymorphisms are screened in our lab:
1. A719G Polymorphism screening by PCR and RFLP.
2. G238C Polymorphism screening by allele specific PCR.
3. G460A Polymorphism screening by PCR and RFLP
|
9 mL Peripheral blood in EDTA (Purple top) tube. Stored at 4°C. |
| B5072 |
GATA1 Mutation screening
Screening of GATA1 Exon2 mutations in Down syndrome associated transient myeloproliferative disorder (TMPD) and myeloid leukemia by DNA Sequencing. |
9 mL Peripheral blood in EDTA (Purple top) tube. Stored at 4°C. |
The following tests are under validation and will be introduced soon in the laboratory
