Dr. Ramachandran Shaji

Dr. Ramachandran Shaji, M.Sc, PhD

  • Globin gene regulation
  • Transcriptional regulation in erythropoiesis
  • Mechanisms of reprogramming

Introduction of four transcription factors Oct-4, Sox2, Klf4 and c-Myc into adult cells can covert them to pluripotent stem cells. These induced pluripotent stem cells (iPSCs) exhibit features characteristic of embryonic stem (ES) cells. These cells have exciting new prospects for biomedical research, drug discovery and for regenerative medicine. Our lab is interested in understanding the mechanism of the reprogramming process and the use of these cells to understand the pathophysiology of human diseases.

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  • Manian KV, Bharathan SP, Maddali M, Srivastava VM, Srivastava A, Shaji RV. Generation of an integration-free iPSC line (CSCRi005-A) from erythroid progenitor cells of a healthy Indian male individual. Stem Cell Res. 2018 Apr 3;29:148-151.
  • Fouzia NA, Edison ES, Lakshmi KM, Korula A, Shaji RV, Balasubramanian P, Abraham A, Viswabandya A, George B, Mathews V, Srivastava A. Long-term outcome of mixed chimerism after stem cell transplantation for thalassemia major conditioned with busulfan and cyclophosphamide. Bone Marrow Transplant. 2018 Feb;53(2):169-174.
  • Karathedath S, Rajamani BM, Musheer Aalam SM, Abraham A, Varatharajan S, Krishnamurthy P, Mathews V, Shaji RV, Balasubramanian P. Role of NF-E2 related factor 2 (Nrf2) on chemotherapy resistance in acute myeloid leukemia (AML) and the effect of pharmacological inhibition of Nrf2. PLoS One. 2017 May 15;12(5):e0177227.
  • Sumitha Prameela Bharathana, Krittika Nandy, Dhavapriya Palani, Nancy Beryl Janet A, Kasthuri Natarajan, Biju George, Alok Srivastava, Shaji RV. Generation of an induced pluripotent stem cell line that mimics the disease phenotypes from a patient with Fanconi anemia by conditional complementation. Stem Cell Research Volume 20, April 2017, Pages 54–57.
  • Sumitha Prameela Bharathan, Kannan Vrindavan Manian, Syed Mohammed Musheer Aalam, Dhavapriya Palani, Prashant Ajit Deshpande, Mankuzhy Damodaran Pratheesh, Alok Srivastava, Shaji RV. Systematic evaluation of markers used for the identification of human induced pluripotent stem cells. Biology Open 2017 6: 100-108; doi: 10.1242/bio.022111.
  • Srivastava A,Shaji RV. Cure for thalassemia major: from allogeneic hematopoietic stem cell transplantation to gene therapy. Haematologica 2017 Feb;102(2):214-223.
  • Aalam SM, Manian KV, Bharathan SP, Mayuranathan T, Shaji RV. Identification of Stable OCT4+NANOG- State in Somatic Cell Reprogramming. Cell Reprogram. 2016 Nov;18(6):367-368.
  • Deshpande P, Kathirvel K, Alex AA, Korula A, George B, Shaji RV, Mathews V. Leukocyte Adhesion Deficiency-I: Clinical and Molecular Characterization in an Indian Population. Indian J Pediatr. 2016 Aug;83(8):799-804.
  • Kamath MS, Pradhan S, Edison ES, Shaji RV, Antonisamy B, Karthikeyan M, Mangalaraj AM, Kunjummen A, George K. Chorionic villous sampling through transvaginal ultrasound approach: A retrospective analysis of 1138 cases. J Obstet Gynaecol Res. 2016 Oct;42(10):1229-1235.
  • Pal R, Mariappan I, Shaji RV. Editorial: Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells: Ushering of a New Era in Personalized Cell Therapies. Curr Stem Cell Res Ther. 2016;11(2):97-8.
  • Manian KV, Aalam SM, Bharathan SP, Srivastava A, Shaji RV. Understanding the Molecular Basis of Heterogeneity in Induced Pluripotent Stem Cells. Cellular Reprogramming 2015 Dec;17(6):427-40.
  • Jayasree D, Shaji RV, George B, Mathews V, Srivastava A, Edison ES. Clinical, Hematological and Molecular Analysis of Homozygous Hb E (HBB: c.79G?>?A) in the Indian Population. Hemoglobin. 2016;40(1):16-9.
  • Abraham A, Varatharajan S, Karathedath S, Philip C, Lakshmi KM, Jayavelu AK, Mohanan E, Janet NB, Srivastava VM, Shaji RV, Zhang W, Abraham A, Viswabandya A, George B, Chandy M, Srivastava A, Mathews V, Balasubramanian P. RNA expression of genes involved in cytarabine metabolism and transport predicts cytarabine response in acute myeloid leukemia. Pharmacogenomics. 2015 Jul;16(8):877-90.
  • Deshpande P, Kamalanathan N, Sampath E, George B, Shaji RV, Edison ES. Characterization of Clinical and Laboratory Profiles of the Deletional a2-Globin Gene Polyadenylation Signal Sequence (AATAAA?>?AATA-?-) in an Indian Population. Hemoglobin. 2015 Dec;39(6):415-8.
  • Mayuranathan T, Rayabaram J, Das R, Arora N, Edison ES, Chandy M, Srivastava A, Shaji RV. Identification of rare and novel deletions that cause (dß)0-thalassaemia and hereditary persistence of foetal haemoglobin in Indian population. Eur J Haematol. 2014 Jun;92(6):514-20.
  • Mayuranathan T, Rayabaram J, Edison ES, Srivastava A, Shaji RV. A novel deletion of ß-globin promoter causing high HbA2 in an Indian population Haematologica. 2012 Sep;97(9):1445-7.
  • David S, Jayandharan GR, Abraham A, Jacob RR, Devi GS, Patkar N, Shaji RV, Nair SC, Viswabandya A, Ahmed R, George B, Mathews V, Chandy M, Srivastava A. Molecular basis of Wiskott-Aldrich syndrome in patients from India. Eur J Haematol. 2012 Oct;89(4):356-60.
  • Varatharajan S, Abraham A, Zhang W, Shaji RV, Ahmed R, Abraham A, George B, Srivastava A, Chandy M, Mathews V, Balasubramanian P. Carbonyl reductase 1 expression influences daunorubicin metabolism in acute myeloid leukemia. Eur J Clin Pharmacol. 2012 Dec;68(12):1577-86.
  • Jain S, Edison ES, Mathews V, Shaji RV. A novel d-globin gene mutation (HBD: c.323G>A) masking the diagnosis of ß-thalassemia: a first report from India. Int J Hematol. 2012 May;95(5):570-2.
  • Edison ES, Sathya M, Rajkumar SV, Nair SC, Srivastava A, Shaji RV. A novel ß-globin gene mutation HBB.c.22 G>C produces a hemoglobin variant (Hb Vellore) mimicking HbS in HPLC. Int J Lab Hematol. 2012 Oct;34(5):556-8.
  • Abraham A, Varatharajan S, Abbas S, Zhang W, Shaji RV, Ahmed R, Abraham A, George B, Srivastava A, Chandy M, Mathews V, Balasubramanian P. Cytidine deaminase genetic variants influence RNA expression and cytarabine cytotoxicity in acute myeloid leukemia. Pharmacogenomics. 2012 Feb;13(3):269-82.
  • Edison ES, Venkatesan RS, Govindanattar SD, George B, Shaji RV. A novel 26 bp deletion [HBB: c.20_45del26bp] in exon 1 of the ß-globin gene causing ß-thalassemia major. Hemoglobin.2012;36(1):98-102.
  • Edison ES, Shaji RV, Chandy M, Srivastava A. Interaction of hemoglobin E with other abnormal hemoglobins. Acta Haematol. 2011;126(4):246-8.
  • Mathews V, George B, Chendamarai E, Lakshmi KM, Desire S, Balasubramanian P, Viswabandya A, Thirugnanam R, Abraham A, Shaji RV, Srivastava A, Chandy M. Single-agent arsenic trioxide in the treatment of newly diagnosed acute promyelocytic leukemia: long-term follow-up data. J Clin Oncol. 2010 Aug 20;28(24):3866-71.



Degree Year
MSc Biotechnology 1991-1993
PhD 1994-2000