Cytogenetics

Test Code	Test	Specimen Requirement
B5029	Conventional cytogenetics- karyotyping of bone marrorw for AML,ALL,MDS,MM,CLL,CML,MPN	VOLUME:At least 1ml of bone marrow (early aspirate) Samples should be sterile& fresh Do not freeze/fix Label tube with patient details & nature of sample Request form with patient details & clinician contact number to be sent with sample contact number to be sent with sample CONTAINER Sodium heparin vacutainer (green top) TIME 8am-3pm: Mon-Fri Before 11am: Sat
B5030	Fluorescence insitu hybridization (FISH)- for specific abnormalities including translocations, deletions, inversion, chimerism ( sex- mismatched) List of available probes Chronic myeloid leukemia : BCR/ABL1 Acute promyelocytic leukemia : PML/RARα, RARα Acute myeloid leukemia / myelodysplastic syndrome: CBFß,RUNX1/RUNX1T1, D7S522, EVI1,EGR1, D20S108,CEP 8 Acute lymphoblastic leukemia: BCR/ABL1, ETV6/RUNX1, MLL,TCF3,IgH,CDKN2A Multiple myeloma : IgH/FGFR3, IgH/CCND1,TP53,D13S319 Chronic lymphocytic leukemia : CEP 12, ATM,TP53,D13S319 Lymphoma : CMYC,BCL2,BCL6 Hypereosinophilic syndrome :PDGFRα Chimersism : CEPXY	VOLUME: 3-5 ml of peripheral blood At least 1ml of bone marrow (early aspirate) Samples should be sterile& fresh Do not freeze/fix Label tube with patient details & nature of sample Request form with patient details & clinician contact number to be sent with sample CONTAINER Sodium heparin vacutainer (green top) TIME 8am-3pm: Mon-Fri Before 11am: Sat
B5037	Chromosome breakage studies for the diagnosis of Fanconi anemia	VOLUME: 3-5 ml of peripheral blood At least 1ml of bone marrow (early aspirate) Samples should be sterile& fresh Do not freeze/fix Label tube with patient details & nature of sample Request form with patient details & clinician contact number to be sent with sample CONTAINER Sodium heparin vacutainer (green top) TIME 8am-3pm: Mon-Fri Before 11am: Sat